CD44 antibody [MEM-263]

Cat No. GTX29524

Host	Mouse
Clonality	Monoclonal
Clone Name	MEM-263
Isotype	IgG1
Application	WB, FACS, IP
Reactivity	Human, Dog, Pig

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Datasheet

APPLICATION

Application Note

FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

82 kDa. ( Note )

Specificity/Sensitivity

The antibody reacts with extracellular (N-terminal) domain of CD44 antigen (Phagocyte glycoprotein 1).

PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

COS-7 cells (African Green monkey).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cd44 Molecule (Indian Blood Group),Cdw44,Cspg8,Ecmr-Iii,Hcell,Hutch-I,In,Lhr,Mc56,Mdu2,Mdu3,Mic4,Pgp1,Cd44

Background

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Database

Gene ID: 960
UniProt: P16070

Research Area

DATA IMAGES

GTX29524 WB Image

Western Blot analysis (non-reducing conditions) of isolated peripheral blood lymphocytes of various species using anti-CD44 (MEM-263) (GTX29524)
Lane 1: lysate of human PBL
Lane 2: lysate of canine PBL
Lane 3: lysate of porcine PBL