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CD44 antibody [N1N2], N-term

KO/KD Comparison
Anti-CD44 antibody [N1N2], N-term used in Western Blot (WB). GTX112893
Anti-CD44 antibody [N1N2], N-term used in Western Blot (WB). GTX112893
Anti-CD44 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112893
Anti-CD44 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112893
Anti-CD44 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112893

Cat. No. GTX112893

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CD44. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949930

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMR-III , HCELL , HUTCH-I , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1

Cellular Localization

Membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [N1N2], N-term used in Western Blot (WB). GTX112893

GTX112893 WB Image

KO/KD

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD44 antibody [N1N2], N-term used in Western Blot (WB). GTX112893

GTX112893 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-CD44 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112893

GTX112893 IHC-P Image

CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD44 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112893

GTX112893 IHC-P Image

CD44 antibody [N1N2], N-term detects CD44 protein at cell membrane on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
CD44 antibody [N1N2], N-term (GTX112893) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-CD44 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX112893

GTX112893 IHC-P Image

The data was published in the journal PLoS One in 2014. PMID: 25337817

REFERENCE

Application Reference

Chao YH et al. PLoS One 2014; 9 (10) : e110338 An increase in CD3+CD4+CD25+ regulatory T cells after administration of umbilical cord-derived mesenchymal stem cells during sepsis.
Application : IHC-P
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169