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CD44 antibody [SPM544]

Anti-CD44 antibody [SPM544] used in IHC (Paraffin sections) (IHC-P). GTX34514
Anti-CD44 antibody [SPM544] used in Western Blot (WB). GTX34514

Cat. No. GTX34514

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM544

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Monkey, Baboon
Package
100 μg ($529)
See all CD44 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Product Note

This antibody's epitope is resistant to digestion by trypsin and chymotrypsin. This MAb selectively interferes with lymphocyte binding to lymph node, mucosal and synovial endothelium.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Stimulated human leukocytes

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD44 , CD44 molecule (Indian blood group) , CDW44 , CSPG8 , ECMRIII , HCELL , HUTCHI , IN , LHR , MC56 , MDU2 , MDU3 , MIC4 , Pgp1 , CD44v3 , CD44 variant 3 , CD44v4 , CD44 variant 4 , CD44v6 , CD44 variant 6 , CD44v7 , CD44 variant 7 , CD44v8 , CD44 variant 8 , CD44v10 , CD44 variant 10

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD44 antibody [SPM544] used in IHC (Paraffin sections) (IHC-P). GTX34514

GTX34514 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX34514 CD44 antibody [SPM544].

Anti-CD44 antibody [SPM544] used in Western Blot (WB). GTX34514

GTX34514 WB Image

WB analysis of HeLa cell lysate using GTX34514 CD44 antibody [SPM544].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529