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CD44 antibody

Anti-CD44 antibody used in Western Blot (WB). GTX131669
Anti-CD44 antibody used in Western Blot (WB). GTX131669

Cat No. GTX131669

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Positive Control

H1299 , HeLa

Specificity/Sensitivity

IP/MS validation was supported by references (PMID:30377419)

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CD44. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd44 Molecule (Indian Blood Group),Cdw44,Cspg8,Ecmr-Iii,Hcell,Hutch-I,In,Lhr,Mc56,Mdu2,Mdu3,Mic4,Pgp1,Cd44

Cellular Localization

Membrane

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CD44 antibody used in Western Blot (WB). GTX131669

GTX131669 WB Image

Wild-type (WT) and CD44 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX131669) diluted at 1:1000.

Anti-CD44 antibody used in Western Blot (WB). GTX131669

GTX131669 WB Image

CD44 antibody detects CD44 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD44 antibody (GTX131669) at a dilution of 1:1000.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169