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CD44v4 antibody [CD44v4/1219]

Anti-CD44v4 antibody [CD44v4/1219] used in IHC (Paraffin sections) (IHC-P). GTX34520
Anti-CD44v4 antibody [CD44v4/1219] used in IHC (Paraffin sections) (IHC-P). GTX34520

Cat No. GTX34520

Host Mouse
Clonality Monoclonal
Clone Name CD44v4/1219
Isotype IgG2a
Application ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

82 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fragment corresponding to the v4 domain of human CD44 (exact sequence is proprietary)

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd44 Molecule (Indian Blood Group),Cdw44,Cspg8,Ecmr-Iii,Hcell,Hutch-I,In,Lhr,Mc56,Mdu2,Mdu3,Mic4,Pgp1,Cd44

Background

The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-CD44v4 antibody [CD44v4/1219] used in IHC (Paraffin sections) (IHC-P). GTX34520

GTX34520 IHC-P Image

Formalin-paraffin human Cervical Squamous Cell Carcinoma stained with CD44v4 Monoclonal Antibody (CD44v4/1219)

Anti-CD44v4 antibody [CD44v4/1219] used in IHC (Paraffin sections) (IHC-P). GTX34520

GTX34520 IHC-P Image

Formalin-paraffin human Tongue Squamous Cell Carcinoma stained with CD44v4 Monoclonal Antibody (CD44v4/1219)

Package List Price ($)
$ 329