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CD45 antibody [2D1] (FITC)

Anti-CD45 antibody [2D1] (FITC) used in Flow cytometry (FACS). GTX01462-06

Cat. No. GTX01462-06

Reference 4

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D1

Isotype

 IgG1

Application

 FACS

Reactivity

 Human
Package
100 test ($319)
See all CD45 products
PRODUCT

Summary

The 2D1 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and CD45RO.The 2D1 antibody is widely used as a marker for human CD45 expression on peripheral blood T cells, B cells, monocytes, macrophages, and NK cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS 1 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , LCA , LY5 , PTPRC , T200 , protein tyrosine phosphatase, receptor type C , CD45.1 , CD45.2 , CD45RA , CD45RB , CD45RC

Cellular Localization

Membrane,Membrane raft

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-CD45 antibody [2D1] (FITC) used in Flow cytometry (FACS). GTX01462-06

GTX01462-06 FACS Image

FACS analysis of human PBMCs using GTX01462-06 CD45 antibody [2D1] (FITC).
Right panel : primary antibody
Left panel : isotype control
antibody amount : 1 μg (5 μl)

REFERENCE

Clone Reference

Zhen Wang et al. Stem Cells Int 2021; 2021:9947187 Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells.
2014; Monocyte-derived dendritic cells from Crohn's disease patients exhibit decreased ability to activate T helper type 17 responses in memory cells.
2010; B cell subsets in healthy children: reference values for evaluation of B cell maturation process in peripheral blood.
1980; Subpopulations of normal and leukemic human thymocytes: an analysis with the use of monoclonal antibodies.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319