PRODUCT
Summary
The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody specifically reacts with all isoforms of CD45 and also with the alloantigens CD45.1 and CD45.2 (LCA).
PROPERTIES
Form
Liquid
Buffer
10mM NaH₂PO₄, 150mM NaCl
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
0.5 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Mouse thymus or spleen
Purification
Purified by affinity chromatography
From tissue culture supernatant
Conjugation
Biotin
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-CD45 antibody [30-F11] (Biotin) used in Flow cytometry (FACS). GTX00456](/templates/default/js/lightbox/images/loading.gif "Anti-CD45 antibody [30-F11] (Biotin) used in Flow cytometry (FACS). GTX00456")
|
GTX00456 FACS Image
FACS analysis of C57Bl/6 splenocytes using GTX00456 CD45 antibody [30-F11] (Biotin).
Solid line : Primary antibody
Dashed line : Biotin rat IgG2b isotype control
Antibody amount : 0.25 μg
|
REFERENCE
There are currently no references for CD45 antibody [30-F11] (Biotin) (GTX00456). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD45 antibody [30-F11] (Biotin) (GTX00456). Be the first to share your experience with this product.
![Anti-CD45 antibody [30-F11] (Biotin) used in Flow cytometry (FACS). GTX00456](/upload/website/prouct_img/small/GTX00456/GTX00456_20201020_FACS_18_w_23053121_885.webp "Anti-CD45 antibody [30-F11] (Biotin) used in Flow cytometry (FACS). GTX00456")
![Anti-CD45 antibody [30-F11] (Biotin) used in Flow cytometry (FACS). GTX00456](/upload/website/prouct_img/normal/GTX00456/GTX00456_20201020_FACS_18_w_23053121_885.webp "Anti-CD45 antibody [30-F11] (Biotin) used in Flow cytometry (FACS). GTX00456")