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CD45 antibody [F10-89-4] (Biotin)

Cat. No. GTX76582

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 F10-89-4

Isotype

 IgG2a

Application

 FACS, IHC

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all CD45 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
FACS Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Product Note

This antibody reacts with all forms of CD45.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human T lymphocytes.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Biotin

RRID

AB_377431

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Database

Research Area

REFERENCE

Application Reference

Yueh MF et al. Proc Natl Acad Sci U S A 2014; 111 (48) : 17200-5 The commonly used antimicrobial additive triclosan is a liver tumor promoter.
Application : IHC
Reactivity : Mouse
Toti P et al. Am J Reprod Immunol 2011; 65 (5) : 470-9 Focal increases of fetal macrophages in placentas from pregnancies with histological chorioamnionitis: potential role of fibroblast monocyte chemotactic protein-1.

Clone Reference

Cardenas I et al. J Immunol 2011; 187 (2):980-6 Nod1 activation by bacterial iE-DAP induces maternal-fetal inflammation and preterm labor.
Mulla MJ et al. Am J Reprod Immunol 2011; 65 (6):542-8 Uric acid induces trophoblast IL-1β production via the inflammasome: implications for the pathogenesis of preeclampsia.
Tang Z et al. Am J Reprod Immunol 2011; 66 (4):336-48 Isolation of hofbauer cells from human term placentas with high yield and purity.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399