CD45RA antibody [GT0014]

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody (GTX628507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [GT0014] (GTX628507) diluted at 1:1000.

Raji whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [GT0014] (GTX628507) diluted at 1:1000.

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [GT0014] (GTX628507) diluted at 1:1000.

CD45RA antibody [GT0014] detects CD45RA protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
CD45RA stained by CD45RA antibody [GT0014] (GTX628507) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Corresponding RNA levels (nTPM) in the tissues are based on Human Protein Atlas program.

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in ice-cold MeOH for 5 min.
Green: CD45RA protein stained by CD45RA antibody [GT0014] (GTX628507) diluted at 1:500.
Blue: Hoechst 33342 staining.

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein by flow cytometry analysis.
Sample: THP-1 cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Black: Isotype control dilution: 1:100.
Green: CD45 antibody [GT0014] (GTX628507) dilution: 1:100.

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD45RA antibody [GT0014] (GTX628507) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

CD45RA antibody [GT0014] (GTX628507) detects CD45RA protein by flow cytometry analysis.
Sample: mouse splenocytes cell.
Left: Unlabelled sample was used as a control.
Right: CD45RA antibody [GT0014] (GTX628507) dilution: 1:50.
Acquisition of 40,000 events were collected for FACS analysis.