GeneTex
United States (US)

CD45 antibody [GT0014]

Orthogonal
GTX628507_41219_20151228_FACS_18121411_703.jpg
GTX628507_41666_IFA_18121411_571.jpg
GTX628507_41218_20150910_FACS_18121411_582.jpg
GTX628507_41218_20170119_WB_Fraction_18121411_530.jpg
GTX628507_41218_FACS_18121411_359.jpg

Cat No. GTX628507

Host Mouse
Clonality Monoclonal
Clone Name GT0014
Isotype IgG1
Application WB, ICC/IF, FACS
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
FACS 1:1:50-1:200
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

Raji , Raji membrane extract
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The immunogen used to generate this antibody corresponds to human CD45

Purification

Affinity purified by Protein G.
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Protein Tyrosine Phosphatase, Receptor Type C,B220,Cd45,Cd45R,Gp180,L-Ca,Lca,Ly5,T200,Ptprc

Cellular Localization

Membrane

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX628507_41219_20151228_FACS_18121411_703.jpg

GTX628507 FACS Image

CD45 antibody [GT0014] (GTX628507) detects CD45 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD45 antibody [GT0014] (GTX628507) dilution: 1:50.
Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX628507_41666_IFA_18121411_571.jpg

GTX628507 ICC/IF Image

CD45 antibody [GT0014] detects CD45 protein at cell membrane by immunofluorescent analysis.
Sample: Raji cells were fixed in ice-cold MeOH for 5 min.
Green: CD45 protein stained by CD45 antibody [GT0014] (GTX628507) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX628507_41218_20150910_FACS_18121411_582.jpg

GTX628507 FACS Image

CD45 antibody [GT0014] (GTX628507) detects CD45 protein by flow cytometry analysis.
Sample: mouse splenocytes cell.
Left: Unlabelled sample was used as a control.
Right: CD45 antibody [GT0014] (GTX628507) dilution: 1:50.
Acquisition of 40,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.

GTX628507_41218_20170119_WB_Fraction_18121411_530.jpg

GTX628507 WB Image

Orthogonal

Raji whole cell and membrane extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [GT0014] (GTX628507) diluted at 1:1000.
The observed M.W. is based on the publication: PMID: 24405902

GTX628507_41218_FACS_18121411_359.jpg

GTX628507 FACS Image

CD45R antibody detects CD45R protein by flow cytometry analysis.
Sample: THP-1 cell fixed in 4% paraformaldehyde at 4ºC for 15 min.
Black: Isotype control (ab97239) dilution: 1:100.
Green: CD45R antibody (GTX628507) dilution: 1:100.

REFERENCE
SDS
PBS.pdf
Package List Price ($)
$ 319
$ 169