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CD45 antibody [HI30] (PerCP-Cy5.5)

Anti-CD45 antibody [HI30] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX00526-11

Cat No. GTX00526-11

Host

Mouse

Clonality

Monoclonal

Clone Name

HI30

Isotype

IgG1

Application

FACS

Reactivity

Human, Chimpanzee
Package
100 test ($339)
PRODUCT

Summary

The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
FACS 0.25 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Specificity/Sensitivity

This antibody recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen).

PROPERTIES

Form

Liquid

Buffer

10 mM NaH₂PO₄ (pH 7.2), 150 mM NaCl, 0.09% sodium azide, 0.1% gelatin

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.05 mg/ml

Antigen Species

Human

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Peridinin-chlorophyll proteins-Cyanine5.5 (PerCP-Cy5.5)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-CD45 antibody [HI30] (PerCP-Cy5.5) used in Flow cytometry (FACS). GTX00526-11

GTX00526-11 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX00526-11 CD45 antibody [HI30] (PerCP-Cy5.5).
Solid line : Primary antibody
Dashed line : PerCP-Cy5.5 mouse IgG1 isotype control
Antibody amount : 0.25 μg

REFERENCE

REVIEW

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Clone Reference
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339