CD45 antibody [HL5696]

CD45 antibody [HL5696] detects CD45 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human tissues.
CD45 stained by CD45 antibody [HL5696] (GTX646527) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD45 antibody [HL5696] detects CD45 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human appendix.
CD45 stained by CD45 antibody [HL5696] (GTX646527) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CD45 antibody [HL5696] detects CD45 protein by immunofluorescent analysis.
Sample: Daudi cells were fixed in 4% paraformaldehyde at RT for 15 min and treated with Fc blocker after blocking step.
Green: CD45 stained by CD45 antibody [HL5696] (GTX646527) diluted at 1:500.
Blue: Fluoroshield™ with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody [HL5696] (GTX646527) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.

CD45 antibody [HL5696] detects CD45 protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD45 stained by CD45 antibody [HL5696] (GTX646527) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).