GeneTex
United States (US)

CD45 antibody [MEM-28]

CD45-antibody-MEM-28-GTX28216-FACS-1_18121409_536.jpg
CD45-antibody-MEM-28-GTX28216-IHC-1_18121409_160.jpg
CD45-antibody-MEM-28__GTX28216-1_18121409_723.gif

Cat No. GTX28216

Host Mouse
Clonality Monoclonal
Clone Name MEM-28
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP, IHC
Reactivity Human
APPLICATION

Application Note

For IHC-P: Use at an assay dependent dilution. No pre-treatment of tissue sections is essential. For WB: Use at a concentration of 2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

147 kDa. ( Note )

Positive Control

Jurkat human leukemia T-cell lysate and Kg-1a human leukemia T-cell lysate

Specificity/Sensitivity

This product reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline, pH 7.4, containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LY5, CD45, T200, 5788, PTPRC, LCA, P08575, B220, 126200, GP180

Cellular Localization

Type I membrane protein

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.

Database

Research Area

DATA IMAGES
CD45-antibody-MEM-28-GTX28216-FACS-1_18121409_536.jpg

GTX28216 FACS Image

Surface staining of human peripheral blood cells with anti-human CD45 [MEM-28] (APC).

CD45-antibody-MEM-28-GTX28216-IHC-1_18121409_160.jpg

GTX28216 IHC Image


CD45-antibody-MEM-28__GTX28216-1_18121409_723.gif

GTX28216 ICC/IF Image

Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28 (GTX28216), green). DNA visualized by DAPI (blue)

Package List Price ($)
$ 319