For IHC-P: Use at an assay dependent dilution. No pre-treatment of tissue sections is essential. For WB: Use at a concentration of 2 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.
Jurkat human leukemia T-cell lysate and Kg-1a human leukemia T-cell lysate
This product reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Phosphate buffered saline, pH 7.4, containing 15mM sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).
Protein A purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
LY5, CD45, T200, 5788, PTPRC, LCA, P08575, B220, 126200, GP180
Type I membrane protein
CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions.
GTX28216 FACS Image
Surface staining of human peripheral blood cells with anti-human CD45 [MEM-28] (APC).
GTX28216 IHC Image
GTX28216 ICC/IF Image
Immunocytochemistry staining of human peripheral blood mononuclear cell using anti-human CD45 (MEM-28 (GTX28216), green). DNA visualized by DAPI (blue)