CD45 antibody

Cat. No. GTX116018

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Human, Mouse, Rat, Rabbit
Package	100 μl ($419), 25 μl ($169)

See all CD45 products

View product citations for antibody GTX116018 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	Assay dependent
IHC-Fr	Assay dependent
FACS	1:50-1:200

Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

Raji , Raji membrane fraction extract

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CD45. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10621655

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq]

Database

Gene ID: 5788 PTPRC
UniProt: P08575 PTPRC

Research Area

DATA IMAGES

GTX116018 FACS Image

CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis.
Sample: THP-1 cell.
Black: Unlabelled sample was used as a control.
Red: CD45 antibody (GTX116018) dilution: 1:50.

GTX116018 ICC/IF Image

CD45 antibody detects CD45 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CD45 protein stained by CD45 antibody (GTX116018) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX116018 FACS Image

CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis.
Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4oC for 15 min.
Black: Unlabelled sample was used as a control.
Red: CD45 antibody (GTX116018) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

GTX116018 WB Image

Raji whole cell and membrane extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody (GTX116018) diluted at 1:1000.