GeneTex

  • Country / Location Selection

United States (US)

CD45 antibody

Anti-CD45 antibody used in IHC (Paraffin sections) (IHC-P). GTX65913
Anti-CD45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65913
Anti-CD45 antibody used in IHC (Paraffin sections) (IHC-P). GTX65913
Anti-CD45 antibody used in Western Blot (WB). GTX65913
Anti-CD45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65913
Anti-CD45 antibody used in IHC (Paraffin sections) (IHC-P). GTX65913

Cat. No. GTX65913

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD45 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human PTPRC

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-CD45 antibody used in IHC (Paraffin sections) (IHC-P). GTX65913

GTX65913 IHC-P Image

IHC-P analysis of mouse lung tissue using GTX65913 CD45 antibody.

Anti-CD45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65913

GTX65913 ICC/IF Image

ICC/IF analysis of Raw264.7 cells using GTX65913 CD45 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD45 antibody used in IHC (Paraffin sections) (IHC-P). GTX65913

GTX65913 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX65913 CD45 antibody.

Anti-CD45 antibody used in Western Blot (WB). GTX65913

GTX65913 WB Image

WB analysis of mouse thymus tissue lysate using GTX65913 CD45 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-CD45 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX65913

GTX65913 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX65913 CD45 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD45 antibody used in IHC (Paraffin sections) (IHC-P). GTX65913

GTX65913 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX65913 CD45 antibody.

REFERENCE

Application Reference

In?s C M Sim?es et al. Int J Mol Sci 2021; 22 (13) The Alterations of Mitochondrial Function during NAFLD Progression-An Independent Effect of Mitochondrial ROS Production.
Application : IHC-P
Reactivity : Mouse
Hou B et al. Cell Death Dis 2020; 11 (5) : 377 Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype.
Submit a Reference

REVIEW

There are currently no reviews for CD45 antibody (GTX65913). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399