CD45R antibody [RA3-6B2] (FITC)

Cat No. GTX16185

Host	Rat
Clonality	Monoclonal
Clone Name	RA3-6B2
Isotype	IgG2a
Application	ICC/IF, FACS
Reactivity	Mouse

See all CD45R products

Datasheet
Reference

APPLICATION

Application Note

FACS: Use 0.25μg for 10^6 cells. IF: Use at a dilution of 1/1000. Optimal dilutions/concentrations should be determined by the end user.

PROPERTIES

Form

Liquid

Buffer

Preservative: 0.1% Sodium Azide; Constituents: PBS, 1mg/ml BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells.

Purification

Protein G purified

Conjugation

Fluorescein isothiocyanate (FITC)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Cell Membrane

Background

CD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. CD45R also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.