CD45R antibody [RA3-6B2] (FITC)

Cat. No. GTX74796

Host	Rat
Clonality	Monoclonal
Clone Name	RA3-6B2
Isotype	IgG2a
Application	FACS
Reactivity	Human, Mouse, Cat
Package	100 μg ($349)

See all CD45R products

View product citations for antibody GTX74796 on CiteAb

PRODUCT

Summary

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	Assay dependent

Not tested in other applications.

Calculated MW

145 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Murine leukaemia-induced pre-B tumour cells (RAW112).

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Fluorescein isothiocyanate (FITC)

RRID

AB_366774

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase, receptor type, C , B220 , CD45R , Cd45 , L-CA , Ly-5 , Lyt-4 , T200 , loc

Cellular Localization

Cell membrane

Database

Gene ID: 19264 Ptprc
UniProt: P06800 Ptprc

Research Area

DATA IMAGES

GTX74796 FACS Image

FACS analysis of C57Bl/6 splenocytes using GTX74796 CD45R antibody [RA3-6B2] (FITC).
Solid line : Primary antibody
Dashed line : FITC rat IgG2a isotype control
Antibody amount : 0.25 μg