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CD45R antibody [RA3-6B2]

Cat. No. GTX00486

Reference 8

Host

 Rat

Clonality

 Monoclonal

Clone Name

 RA3-6B2

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, Depletion

Reactivity

 Human, Mouse, Cat
Package
100 μg ($399)
See all CD45R products
PRODUCT

Summary

The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function. In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets. It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets. Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
Depletion Assay dependent
Not tested in other applications.

Calculated MW

145 kDa. ( Note )

Product Note

This antibody recognizes an epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase, receptor type, C , B220 , CD45R , Cd45 , L-CA , Ly-5 , Lyt-4 , T200 , loc

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Clone Reference

2012; A role for GPx3 in activity of normal and leukemia stem cells
Application : FACS
Nagashima T et al. Am J Pathol 2011; 178 (1):222-32 Arachidonate 5-lipoxygenase establishes adaptive humoral immunity by controlling primary B cells and their cognate T-cell help.
2011; Loss of Roquin induces early death and immune deregulation but not autoimmunity
Application : FACS
Reactivity : Mouse
2010; Widespread overexpression of epitope-tagged Mdm4 does not accelerate tumor formation in vivo
Application : WB
Reactivity : Mouse
2005; Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors
Application : Depletion
Reactivity : Mouse
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399