CD45RA antibody [HI100] (APC)

Cat. No. GTX01464-07

Host	Mouse
Clonality	Monoclonal
Clone Name	HI100
Isotype	IgG2b
Application	FACS
Reactivity	Human, Chimpanzee
Package	100 test ($259)

See all CD45RA products

View product citations for antibody GTX01464-07 on CiteAb

PRODUCT

Summary

The HI100 antibody reacts with the human CD45 isoform known as CD45RA, a protein tyrosine phosphatase of ≥ 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RB, CD45RO and others. The HI100 antibody is widely used as a marker for human CD45RA expression on naive and activated T cells, B cells, and monocytes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
FACS	0.125 μg (5 μl) for 10⁵-10⁸ cells in 100 μl sample per test

Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM NaH₂PO₄, 150mM NaCl, 0.1% Gelatin

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Concentration

0.025 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Allophycocyanin (APC) Wavelength

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , LCA , LY5 , PTPRC , T200 , protein tyrosine phosphatase, receptor type C , CD45.1 , CD45.2 , CD45RA , CD45RB , CD45RC

Cellular Localization

Membrane,Membrane raft

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Database

Gene ID: 5788 PTPRC
UniProt: P08575 PTPRC

Research Area

DATA IMAGES

GTX01464-07 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX01464-07 CD45RA antibody [HI100] (APC).
Solid lone : primary antibody
Dashed line : isotype control
antibody amount : 0.125 μg (5 μl)