GeneTex
United States (US)

CD45RA antibody [MEM-56]

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CD45RA-antibody-MEM-56__GTX28217-1_18121409_370.jpg

Cat No. GTX28217

Host Mouse
Clonality Monoclonal
Clone Name MEM-56
Isotype IgG2b
Application WB, IHC-P, FACS, IP, IHC
Reactivity Human
APPLICATION

Application Note

For FACS: Use at an assay dependant dilution. For IHC-P: Use at an assay dependant dilution. No pre-treatment of tissue sections is essential. For IP: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the researcher.

Specificity/Sensitivity

CD45RA antigen (human)
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline, pH 7.4, containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tissue / cell preparation (Human). (Thymocytes and T lymphocytes).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Q14761, CD45, LY5, CD45AP, T200, 5788, PTPRCAP, 601577, PTPRC, LCA, CD45RA, P08575, 5790, LPAP, B220, 126200, GP180, CD45-AP, CD45 AP

Background

The CD45 molecule is comprised of different glycoproteins ranging from 180-240 kD. Expression of CD45 is found on all hemopoietic cells. Detection of the different isoforms can distinguish between different cell forms i.e. naive T cells and memory T cells. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. Their functional activity of each CD45 isotype also varies.
DATA IMAGES
img12560_18121411_194.jpg

GTX28217 IHC Image


CD45RA-antibody-MEM-56__GTX28217-1_18121409_370.jpg

GTX28217 WB Image

Western Blot analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line.
Lane 1: immunostaining with anti-human CD45 control antibody
Lane 2: immunostaining with anti-human CD45RA (MEM-56) (GTX28217); sample of cell lysate prepared under non-reducing conditions
Lane 3: immunostaining with anti-human CD45RA (MEM-56) (GTX28217); sample of cell lysate prepared under reducing conditions

Package List Price ($)
$ 319