APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2 μg/ml |
| 1-2 μg/ml |
| 1-2 μg/ml |
| 1-2 μg/million cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA (pH 9.0) for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Observed MW
200-220 kDa. The observed M.W. is based on the following publication. PMID: 16352531
Product Note
We do not recommend use of this product for Rat,Rhesus Monkey samples.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
Concentration
200 μg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human CD45RB protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
protein tyrosine phosphatase receptor type C , B220 , CD45 , CD45R , GP180 , L-CA , LCA , LY5 , T200
Cellular Localization
Cell membrane
Background
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-CD45RB antibody [PTPRC/2877R] used in IHC (Paraffin sections) (IHC-P). GTX02760](/templates/default/js/lightbox/images/loading.gif "Anti-CD45RB antibody [PTPRC/2877R] used in IHC (Paraffin sections) (IHC-P). GTX02760")
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GTX02760 IHC-P Image
IHC-P analysis of human lymph node tissue section using GTX02760 CD45RB antibody [PTPRC/2877R].
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GTX02760 WB Image
WB analysis of Raji whole cell lysate using GTX02760 CD45RB antibody [PTPRC/2877R].
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GTX02760 FACS Image
FACS analysis of PFA-fixed Raji cells using GTX02760 CD45RB antibody [PTPRC/2877R].
Blue : Primary antibody
Red : Isotype control
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GTX02760 ICC/IF Image
ICC/IF analysis of PFA-fixed Raji cells using GTX02760 CD45RB antibody [PTPRC/2877R].
Green : Primary antibody
Red : Phalloiden (membrane)
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GTX02760 WB Image
WB analysis of human spleen tissue lysate using GTX02760 CD45RB antibody [PTPRC/2877R].
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REFERENCE
There are currently no references for CD45RB antibody [PTPRC/2877R] (GTX02760). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD45RB antibody [PTPRC/2877R] (GTX02760). Be the first to share your experience with this product.
![Anti-CD45RB antibody [PTPRC/2877R] used in IHC (Paraffin sections) (IHC-P). GTX02760](/upload/website/prouct_img/small/GTX02760/GTX02760_20210319_IHC-P_w_23053122_535.webp "Anti-CD45RB antibody [PTPRC/2877R] used in IHC (Paraffin sections) (IHC-P). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760](/upload/website/prouct_img/small/GTX02760/GTX02760_20210319_WB_w_23053122_277.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Flow cytometry (FACS). GTX02760](/upload/website/prouct_img/small/GTX02760/GTX02760_20210319_FACS_w_23053122_679.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Flow cytometry (FACS). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02760](/upload/website/prouct_img/small/GTX02760/GTX02760_20210319_ICCIF_w_23053122_953.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760](/upload/website/prouct_img/small/GTX02760/GTX02760_20210319_WB_1_w_23053122_856.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in IHC (Paraffin sections) (IHC-P). GTX02760](/upload/website/prouct_img/normal/GTX02760/GTX02760_20210319_IHC-P_w_23053122_535.webp "Anti-CD45RB antibody [PTPRC/2877R] used in IHC (Paraffin sections) (IHC-P). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760](/upload/website/prouct_img/normal/GTX02760/GTX02760_20210319_WB_w_23053122_277.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Flow cytometry (FACS). GTX02760](/upload/website/prouct_img/normal/GTX02760/GTX02760_20210319_FACS_w_23053122_679.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Flow cytometry (FACS). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02760](/upload/website/prouct_img/normal/GTX02760/GTX02760_20210319_ICCIF_w_23053122_953.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02760")
![Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760](/upload/website/prouct_img/normal/GTX02760/GTX02760_20210319_WB_1_w_23053122_856.webp "Anti-CD45RB antibody [PTPRC/2877R] used in Western Blot (WB). GTX02760")