GeneTex

  • Country / Location Selection

United States (US)

CD45RO antibody [UCHL1] (Biotin)

Cat. No. GTX19741

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 UCHL1

Isotype

 IgG2a

Application

 FACS

Reactivity

 Human
Package
200 μg ($429)
See all CD45RO products

APPLICATION

Application Note

When activated with phytohemagglutinin (PHA), naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. Therefore, Anti-CD45RO may be a marker for memory T-cells. Anti-CD45RO may be used in formalin-fixed and paraffin-embedded tissues as well as frozen sections and cell suspensions. FACS: Use 1μg for 10^6 cells. Optimal dilutions/concentrations should be determined by the end user.

Product Note

Recognizes the low molecular weight (Mr 180kDa) isoform of the leucocyte common antigen (LCA) which is part of a family of protein tryosine phosphatases. The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.2, 1% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

IL-2 dependent T-cell line CA1.

Purification

Protein G purified
Protein G chromatography

Conjugation

Biotin

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Type 1 membrane protein

Background

NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.

Research Area

REFERENCE

There are currently no references for CD45RO antibody [UCHL1] (Biotin) (GTX19741). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for CD45RO antibody [UCHL1] (Biotin) (GTX19741). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 429