CD46 antibody [M177]

Cat. No. GTX54447

Host	Mouse
Clonality	Monoclonal
Clone Name	M177
Isotype	IgG1
Application	WB, IHC-Fr, FACS, IP, ELISA, Functional Assay
Reactivity	Human
Package	50 μg ($399)

See all CD46 products

Datasheet

View product citations for antibody GTX54447 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
Functional Assay	Assay dependent
Note : WB A non-reduced sample treatment and SDS-page was used. IP 10⁷ cells were lysed and immunoprecipitated with 25 μg M177 antibody and 25 μg protein G-sepharose

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

This antibody stains the extracellular domain of CD46. The monoclonal antibody M177 reacts with the SCR2 domain.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 0.1% BSA

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

100 μg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified MCP

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AHUS2,CD46,CD46 molecule,MCP,MIC10,TLX,TRA2.10

Cellular Localization

Cytoplasmic vesicle,Secretory vesicle,Acrosome inner membrane

Background

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

Database

Gene ID: 4179 CD46
UniProt: P15529 CD46

Research Area

DATA IMAGES

GTX54447 FACS Image

FACS analysis of THP-1 cells using GTX54447 CD46 antibody [M177].
Red : Negative control
Black : Cells only control
Blue : Primary antibody
Antibody amount : 10 μg

GTX54447 IHC-Fr Image

IHC-Fr analysis of human kidney tissue sections using GTX54447 CD46 antibody [M177]. The antibody appeared to give a specific staining in glomeruli, juxtaglomerular apparatus and tubuli.
Dilution : 1:4000

REFERENCE

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REVIEW

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Package

List Price ($)

50 μg

$ 399

Qty