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CD47 antibody [B6H12.2]

Anti-CD47 antibody [B6H12.2] used in Flow cytometry (FACS). GTX04817
Anti-CD47 antibody [B6H12.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04817
Anti-CD47 antibody [B6H12.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04817
Anti-CD47 antibody [B6H12.2] used in (). GTX04817
Anti-CD47 antibody [B6H12.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04817
Anti-CD47 antibody [B6H12.2] used in Flow cytometry (FACS). GTX04817

Cat. No. GTX04817

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 B6H12.2

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, in vitro

Reactivity

 Human
Package
100 μg ($529)
See all CD47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2-4 μg/ml
ICC/IF 1-3 μg/ml
FACS 1- 2 μg/10⁶ cells
in vitro Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Intact CD47 Purified from placenta

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD47,CD47 molecule,IAP,MER6,OA3

Cellular Localization

Cell membrane

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-CD47 antibody [B6H12.2] used in Flow cytometry (FACS). GTX04817

GTX04817 FACS Image

FACS analysis of PFA-fixed Jurkat cells using GTX04817 CD47 antibody [B6H12.2].
Blue : Primary antibody
Red : Isotype Control

Anti-CD47 antibody [B6H12.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04817

GTX04817 ICC/IF Image

ICC/IF analysis of PFA-fixed MCF-7 cells using GTX04817 CD47 antibody [B6H12.2].
Green : Primary antibody
Red : Nuclear staining

Anti-CD47 antibody [B6H12.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04817

GTX04817 ICC/IF Image

ICC/IF analysis of PFA-fixed Jurkat cells using GTX04817 CD47 antibody [B6H12.3].
Green : Primary antibody
Red : Nuclear staining

Anti-CD47 antibody [B6H12.2] used in (). GTX04817

GTX04817 Image

SDS-PAGE analysis of GTX04817 CD47 antibody [B6H12.2].
Loading : 2μg

Anti-CD47 antibody [B6H12.2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04817

GTX04817 ICC/IF Image

ICC/IF analysis of live Jurkat cells using GTX04817 CD47 antibody [B6H12.2].

Anti-CD47 antibody [B6H12.2] used in Flow cytometry (FACS). GTX04817

GTX04817 FACS Image

FACS analysis of live Jurkat cells using GTX04817 CD47 antibody [B6H12.2].
Blue : Primary antibody
Red : Isotype Control

REFERENCE

Clone Reference

SB Willingham et al. Proc Natl Acad Sci U S A 2012; 109 (17):6662–6667 The CD47-signal regulatory protein alpha (SIRPa) interaction is a therapeutic target for human solid tumors
Application : in vitro
Submit a Reference

REVIEW

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Package List Price ($)
$ 529