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CD47 antibody [BRIC126]

Cat. No. GTX76615

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BRIC126

Isotype

 IgG2b

Application

 WB, IHC-Fr, FACS, IP, Functional Assay

Reactivity

 Human, Sheep, Bovine, Dog, Pig
Package
200 μg ($469)
See all CD47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr Assay dependent
FACS 1/10
IP Assay dependent
Functional Assay Assay dependent

Note :

WB
Using non-reducing conditions. The CD47 epitope is inactive in reducing conditions. (Refer to PMID: 12153520)

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

TBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human erythrocytes

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377454

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD47 molecule , IAP , MER6 , OA3

Cellular Localization

Cell membrane

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

REFERENCE

Clone Reference

Ahmer M Hameed et al. Sci Rep . 2020; 10 (1):6930 Pharmacologic targeting of renal ischemia-reperfusion injury using a normothermic machine perfusion platform
Application : IHC-Fr
Reactivity : Pig
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 469