GeneTex
United States (US)

CD47 antibody [MEM-122]

CD47-antibody-MEM-122__GTX29089-1_18121409_889.jpg

Cat No. GTX29089

Host Mouse
Clonality Monoclonal
Clone Name MEM-122
Isotype IgM
Application WB, IHC-Fr, FACS
Reactivity Human, Pig, Monkey, Primate
APPLICATION

Application Note

FACS: Use at an assay dependant dilution. IHC-Fr: Use at an assay dependant dilution. WB: Use at a dilution of 1/100. Use in non-reducing conditions. This antibody causes platelet aggregation and ATP release from platelet-rich plasma but not from washed platelets, indicating a complement-dependent reaction. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

35 kDa. ( Note )

Specificity/Sensitivity

Integrin Associated Protein
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

COS-7 cells transfected with human CD47.

Purification

Purified by precipitation methods and size-exclusion chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
CD47-antibody-MEM-122__GTX29089-1_18121409_889.jpg

GTX29089 FACS Image

Surface staining of lysed and washed porcine peripheral blood with purified anti-CD47 (GTX29089) (detection by anti-mouse IgM: FITC).
Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03; GTX78439)
Panel B, C, D - porcine PBMC sample stained with anti-CD47 (GTX29089)
Cells in the granulocyte gate were used for analysis.

Package List Price ($)
$ 319