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CD47 antibody

Anti-CD47 antibody used in Western Blot (WB). GTX04814
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX04814
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX04814

Cat. No. GTX04814

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.05% Proclin300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 20-140 of human CD47 (NP_001768.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD47,CD47 molecule,IAP,MER6,OA3

Cellular Localization

Cell membrane

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-CD47 antibody used in Western Blot (WB). GTX04814

GTX04814 WB Image

WB analysis of various samples using GTX04814 CD47 antibody.
Loading : 25 μg
Dilution : 1:1000

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX04814

GTX04814 IHC-P Image

IHC-P analysis of human prostate tissue using GTX04814 CD47 antibody.
Orange : Primany antibody
Blue : DAPI
Antigen retrieval : High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0)
Dilution : 1:100

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX04814

GTX04814 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX04814 CD47 antibody.
Antigen retrieval : Microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0
Dilution : 1:100

REFERENCE

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REVIEW

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Package List Price ($)
$ 399