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CD47 antibody

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04816
Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04816
Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04816
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX04816

Cat. No. GTX04816

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($429)
See all CD47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 10 μg/ml
IHC-P 6-7 μg/ml
ELISA 1:128000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Product Note

This antibody is expected to recognize all reported isoforms (NP_001768.1; NP_942088.1; NP_001369235.1).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-SNQKTIQPPRK, from the internal region (near C terminus) of the protein sequence according to NP_001768.1; NP_942088.1; NP_001369235.1.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD47,CD47 molecule,IAP,MER6,OA3

Cellular Localization

Cell membrane

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04816

GTX04816 ICC/IF Image

ICC/IF analysis of PFA-fixed A431 cells using GTX04816 CD47 antibody.
Green : Primary antibody
Blue : DAPI
Ve-control : Unimmunized goat IgG
Permeabilization : 0.15% Triton X-100
Dilution : 10 μg/ml

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04816

GTX04816 ICC/IF Image

ICC/IF analysis of PFA-fixed U2OS cells using GTX04816 CD47 antibody.
Green : Primary antibody
Blue : DAPI
Ve-control : Unimmunized goat IgG
Permeabilization : 0.15% Triton X-100
Dilution : 10 μg/ml

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04816

GTX04816 ICC/IF Image

ICC/IF analysis of PFA-fixed THP-1 cells using GTX04816 CD47 antibody.
Green : Primary antibody
Blue : DAPI
Ve-control : Unimmunized goat IgG
Permeabilization : 0.15% Triton X-100
Dilution : 10μg/ml

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX04816

GTX04816 IHC-P Image

IHC-P analysis of human cortex tissue using GTX04816 CD47 antibody.
Right : Primary antibody
Left : No primary antibody
Antigen retrieval : Heat mediated citrate buffer (pH 6)
Dilution : 7μg/ml

REFERENCE

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Package List Price ($)
$ 429