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CD47 antibody

Orthogonal
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912
Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53912
Anti-CD47 antibody used in Western Blot (WB). GTX53912
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912
Anti-CD47 antibody used in Western Blot (WB). GTX53912
Anti-CD47 antibody used in Western Blot (WB). GTX53912
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912

Cat. No. GTX53912

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all CD47 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human CD47 (NP_942088.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887824

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD47 molecule , IAP , MER6 , OA3

Cellular Localization

Cell membrane

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912

GTX53912 IHC-P Image

IHC-P analysis of rat testis tissue using GTX53912 CD47 antibody.
Dilution : 1:100

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53912

GTX53912 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX53912 CD47 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD47 antibody used in Western Blot (WB). GTX53912

GTX53912 WB Image

WB analysis of various sample lysates using GTX53912 CD47 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912

GTX53912 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX53912 CD47 antibody.
Dilution : 1:100

Anti-CD47 antibody used in Western Blot (WB). GTX53912

GTX53912 WB Image

Orthogonal

The data was published in the journal J Neuroinflammation in 2016.PMID: 27095555

Anti-CD47 antibody used in Western Blot (WB). GTX53912

GTX53912 WB Image

Orthogonal

The data was published in the journal J Neurooncol in 2020. PMID: 32020472

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912

GTX53912 IHC-P Image

The data was published in the journal J Neuroinflammation in 2016.PMID: 27095555

REFERENCE

Application Reference

Hsu SPC et al. J Neurooncol 2020; 146 (3) : 417-426 Rapamycin and hydroxychloroquine combination alters macrophage polarization and sensitizes glioblastoma to immune checkpoint inhibitors.
Application : WB
Reactivity : Mouse
Cabrales P et al. Transl Oncol 2019; 12 (4) : 626-632 RRx-001 Acts as a Dual Small Molecule Checkpoint Inhibitor by Downregulating CD47 on Cancer Cells and SIRP-α on Monocytes/Macrophages.
Application : WB
Reactivity : Mouse
Sun FJ et al. J Neuroinflammation 2016; 13 (1) : 85 Downregulation of CD47 and CD200 in patients with focal cortical dysplasia type IIb and tuberous sclerosis complex.
Application : WB, IHC-P
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399