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CD47 antibody

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53912
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912
Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912
Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53912
Anti-CD47 antibody used in Western Blot (WB). GTX53912

Cat No. GTX53912

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:200
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human CD47 (NP_942088.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD47 molecule , IAP , MER6 , OA3

Cellular Localization

Cell membrane

Background

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53912

GTX53912 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX53912 CD47 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912

GTX53912 IHC-P Image

IHC-P analysis of human lung cancer tissue using GTX53912 CD47 antibody.
Dilution : 1:200

Anti-CD47 antibody used in IHC (Paraffin sections) (IHC-P). GTX53912

GTX53912 IHC-P Image

IHC-P analysis of human placenta tissue using GTX53912 CD47 antibody.
Dilution : 1:200

Anti-CD47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53912

GTX53912 ICC/IF Image

ICC/IF analysis of L929 cells using GTX53912 CD47 antibody.
Blue : DAPI
Dilution : 1:100

Anti-CD47 antibody used in Western Blot (WB). GTX53912

GTX53912 WB Image

WB analysis of various sample lysates using GTX53912 CD47 antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf