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CD5 antibody [2B8]

Anti-CD5 antibody [2B8] used in Western Blot (WB). GTX84708
Anti-CD5 antibody [2B8] used in Flow cytometry (FACS). GTX84708
Anti-CD5 antibody [2B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84708
Anti-CD5 antibody [2B8] used in IHC (Paraffin sections) (IHC-P). GTX84708
Anti-CD5 antibody [2B8] used in IHC (Paraffin sections) (IHC-P). GTX84708
Anti-CD5 antibody [2B8] used in Flow cytometry (FACS). GTX84708

Cat. No. GTX84708

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2B8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all CD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD5 molecule , LEU1 , T1

Cellular Localization

Cell membrane; Single-pass type I membrane protein

Background

May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

Database

Research Area

DATA IMAGES

Anti-CD5 antibody [2B8] used in Western Blot (WB). GTX84708

GTX84708 WB Image

WB analysis of HEK293T cells transfected with CD5 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84708 CD5 antibody [2B8].
Loading : 5 ug per lane

Anti-CD5 antibody [2B8] used in Flow cytometry (FACS). GTX84708

GTX84708 FACS Image

FACS analysis of HEK293T cells transfected with either CD5 plasmid(Red) or empty vector control plasmid(Blue) using GTX84708 CD5 antibody [2B8].

Anti-CD5 antibody [2B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84708

GTX84708 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with CD5 plasmid using GTX84708 CD5 antibody [2B8].

Anti-CD5 antibody [2B8] used in IHC (Paraffin sections) (IHC-P). GTX84708

GTX84708 IHC-P Image

IHC-P analysis of human lymphoma tissue using GTX84708 CD5 antibody [2B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD5 antibody [2B8] used in IHC (Paraffin sections) (IHC-P). GTX84708

GTX84708 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX84708 CD5 antibody [2B8].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-CD5 antibody [2B8] used in Flow cytometry (FACS). GTX84708

GTX84708 FACS Image

FACS analysis of Jurkat cells using GTX84708 CD5 antibody [2B8].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519