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CD5 antibody [CD5/2416]

Anti-CD5 antibody [CD5/2416] used in Western Blot (WB). GTX18024
Anti-CD5 antibody [CD5/2416] used in Flow cytometry (FACS). GTX18024
Anti-CD5 antibody [CD5/2416] used in Flow cytometry (FACS). GTX18024
Anti-CD5 antibody [CD5/2416] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18024
Anti-CD5 antibody [CD5/2416] used in IHC (Paraffin sections) (IHC-P). GTX18024
Anti-CD5 antibody [CD5/2416] used in Protein Array (Protein Array). GTX18024

Cat. No. GTX18024

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 CD5/2416

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS, Protein Array

Reactivity

 Human
Package
100 μg ($529)
See all CD5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/10⁶ cells
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells
Protein Array Assay dependent

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CD5 protein (around aa 269-366) (exact sequence is proprietary)

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD5 , CD5 molecule , LEU1 , T1

Cellular Localization

Cell membrane

Background

This gene encodes a member of the scavenger receptor cysteine-rich (SRCR) superfamily. Members of this family are secreted or membrane-anchored proteins mainly found in cells associated with the immune system. This protein is a type-I transmembrane glycoprotein found on the surface of thymocytes, T lymphocytes and a subset of B lymphocytes. The encoded protein contains three SRCR domains and may act as a receptor to regulate T-cell proliferation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2016]

Database

Research Area

DATA IMAGES

Anti-CD5 antibody [CD5/2416] used in Western Blot (WB). GTX18024

GTX18024 WB Image

WB analysis of Jurkat cell lysate using GTX18024 CD5 antibody [CD5/2416].

Anti-CD5 antibody [CD5/2416] used in Flow cytometry (FACS). GTX18024

GTX18024 FACS Image

FACS analysis of Jurkat cells using GTX18024 CD5 antibody [CD5/2416].
Blue : Primary antibody
Red : Isotype control

Anti-CD5 antibody [CD5/2416] used in Flow cytometry (FACS). GTX18024

GTX18024 FACS Image

FACS analysis of Ramos cells using GTX18024 CD5 antibody [CD5/2416].
Blue : Primary antibody
Red : Isotype control

Anti-CD5 antibody [CD5/2416] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18024

GTX18024 ICC/IF Image

ICC/IF analysis of PFA-fixed Ramos cells using GTX18024 CD5 antibody [CD5/2416].
Green : Primary antibody
Red : nucleus

Anti-CD5 antibody [CD5/2416] used in IHC (Paraffin sections) (IHC-P). GTX18024

GTX18024 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX18024 CD5 antibody [CD5/2416].

Anti-CD5 antibody [CD5/2416] used in Protein Array (Protein Array). GTX18024

GTX18024 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using Mouse CD5-Monospecific Monoclonal Antibody (CD5/2416). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529