GeneTex
United States (US)

CD5 antibody [CD5/54/F6]

Anti-CD5 antibody [CD5/54/F6] used in IHC (Paraffin sections) (IHC-P). GTX34526

Cat No. GTX34526

Host Mouse
Clonality Monoclonal
Clone Name CD5/54/F6
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human, Rat, Rabbit, Bovine, Chicken, Guinea pig, Pig, Monkey, Horse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Specificity/Sensitivity

Recognizes a 67kDa transmembrane protein, which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms. Anti-CD5 detection is diagnostic in CLL/SLL within a panel of other B-cell markers, especially one that includes anti-CD23. Anti-CD5 is also very useful in differentiating among mature small lymphoid cell malignancies. In addition, anti-CD5 can be used in distinguishing thymic carcinoma (+) from thymoma (-). Anti-CD5 does not react with granulocytes or monocytes.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide from the intracellular region of human CD5

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd5 Molecule,Leu1,T1,Cd5

Database

Research Area

DATA IMAGES
Anti-CD5 antibody [CD5/54/F6] used in IHC (Paraffin sections) (IHC-P). GTX34526

GTX34526 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD5 Monoclonal Antibody (CD5/54/F6)

Package List Price ($)
$ 329