WB analysis of mouse testis or sperm tissue lysate using GTX00743 CD52 antibody.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel

ICC/IF analysis of NIH-3T3 cells using GTX00743 CD52 antibody.
Dilution : 1:100
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

IHC-P analysis of mouse testis tissue section using GTX00743 CD52 antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

WB analysis of wild-type and CD52 deficient mice using GTX00743 CD52 antibody.
Upper : Cauda epididymal lysates (30 μg)
Lower : Sperm lysates (10 μg)
(+/+) : Cd52^+/+
(+/-) : Cd52^+/-
(-/-) : Cd52^-/-
Dilution : 1:1000

WB analysis of various mouse tissue samples using GTX00743 CD52 antibody.
Samples: testes, male reproductive ducts (30 μg), and sperm proteinmouse tissue proteins (10 μg)
Dilution : 1:1000