APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 20 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension |
Not tested in other applications.
Calculated MW
Product Note
This antibody reacts with CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes. And this antibody also reacts with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F).
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia.
Purification
Purified by size-exclusion chromatography
Conjugation
Fluorescein isothiocyanate (FITC)
RRID
AB_11167786
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD53 , CD53 molecule , MOX44 , TSPAN25
Cellular Localization
Cell membrane,Cell junction,Membrane
Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Database
Research Area
DATA IMAGES
![Anti-CD53 antibody [MEM-53] (FITC) used in Flow cytometry (FACS). GTX79940](/templates/default/js/lightbox/images/loading.gif "Anti-CD53 antibody [MEM-53] (FITC) used in Flow cytometry (FACS). GTX79940")
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GTX79940 FACS Image
FACS analysis of human peripheral blood using GTX79940 CD53 antibody [MEM-53] (FITC).
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REFERENCE
There are currently no references for CD53 antibody [MEM-53] (FITC) (GTX79940). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD53 antibody [MEM-53] (FITC) (GTX79940). Be the first to share your experience with this product.
![Anti-CD53 antibody [MEM-53] (FITC) used in Flow cytometry (FACS). GTX79940](/upload/website/prouct_img/small/GTX79940/GTX79940_20191025_AP_006_338_w_23061322_781.webp "Anti-CD53 antibody [MEM-53] (FITC) used in Flow cytometry (FACS). GTX79940")
![Anti-CD53 antibody [MEM-53] (FITC) used in Flow cytometry (FACS). GTX79940](/upload/website/prouct_img/normal/GTX79940/GTX79940_20191025_AP_006_338_w_23061322_781.webp "Anti-CD53 antibody [MEM-53] (FITC) used in Flow cytometry (FACS). GTX79940")