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CD55 antibody [N1C2]

Orthogonal
Anti-CD55 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113170
Anti-CD55 antibody [N1C2] used in Western Blot (WB). GTX113170
Anti-CD55 antibody [N1C2] used in Western Blot (WB). GTX113170

Cat. No. GTX113170

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD55 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.86 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD55. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949932

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD55 molecule (Cromer blood group) , CHAPLE , CR , CROM , DAF , TC

Cellular Localization

Isoform 1: Membrane , Isoform 2: Cell membrane , GPI-anchor

Background

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD55 antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113170

GTX113170 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CD55(GTX113170) antibody at 1:200 dilution.

Anti-CD55 antibody [N1C2] used in Western Blot (WB). GTX113170

GTX113170 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD55 antibody [N1C2] (GTX113170) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-CD55 antibody [N1C2] used in Western Blot (WB). GTX113170

GTX113170 WB Image

The data was published in the journal PLoS One in 2016. PMID: 27486856

REFERENCE

Application Reference

Pei-Suen Tsou et al. J Clin Invest 2022; 132 (11) Soluble CD13 induces inflammatory arthritis by activating the bradykinin receptor B1.
Application : IHC-Fr
Reactivity : Human
Nashine S et al. PLoS One 2016; 11 (8) : e0159828 Differential Expression of Complement Markers in Normal and AMD Transmitochondrial Cybrids.
Application : WB
Reactivity : Human
Pan J et al. J Virol 2015; 89 (8) : 880813 Expression of human decay-accelerating factor on intestinal epithelium of transgenic mice does not facilitate infection by the enteral route.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169