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CD55 antibody [N2C3]

Anti-CD55 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103951
Anti-CD55 antibody [N2C3] used in Western Blot (WB). GTX103951

Cat No. GTX103951

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

K562 , THP-1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD55. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD55 molecule (Cromer blood group) , CHAPLE , CR , CROM , DAF , TC

Cellular Localization

Isoform 1: Membrane , Isoform 2: Cell membrane , GPI-anchor

Background

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-CD55 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103951

GTX103951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CD55(GTX103951) antibody at 1:200 dilution.

Anti-CD55 antibody [N2C3] used in Western Blot (WB). GTX103951

GTX103951 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD55 antibody [N2C3] (GTX103951) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf