GeneTex
United States (US)

CD55 antibody [N2C3]

GTX103951_40058_IFA_19011506_375.jpg
GTX103951_40058_WB_19011506_590.jpg

Cat No. GTX103951

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

K562 , THP-1
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD55. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CR antibody, CROM antibody, DAF antibody, TC antibody, CD55 antibody, CD55 antigen antibody, decay accelerating factor for complement antibody, complement decay-accelerating factor antibody, "CD55 molecule, decay accelerating factor for complement (Cromer blood group) antibody"

Cellular Localization

Isoform 1: Membrane , Isoform 2: Cell membrane , GPI-anchor

Background

This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF); binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX103951_40058_IFA_19011506_375.jpg

GTX103951 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using CD55(GTX103951) antibody at 1:200 dilution.

GTX103951_40058_WB_19011506_590.jpg

GTX103951 WB Image

Sample (30 ug of whole cell lysate)

A: THP-1

10% SDS PAGE

GTX103951 diluted at 1:1000

Reference
SDS
Glycerol.pdf
Thimerosal.pdf