GeneTex
United States (US)

CD58 antibody [TS2/9]

GTX15669_285_ICC-IF_19011519_761.jpg
GTX15669_103_FACS_19011519_105.jpg
GTX15669_101_FACS_19011519_126.jpg
GTX15669_1514_WB_19011519_925.jpg

Cat No. GTX15669

Host Mouse
Clonality Monoclonal
Clone Name TS2/9
Isotype IgG1
Application WB, ICC/IF, IHC-Fr, FACS, IP, ELISA, IHC, Neut
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:500
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Neut Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.98 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Leukocyte Function Antigen-3

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd58 Molecule,Lfa-3,Lfa3,Ag3,Cd58

Cellular Localization

Cell membrane

Background

This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a ligand of the T lymphocyte CD2 protein, and functions in adhesion and activation of T lymphocytes. The protein is localized to the plasma membrane. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009]

Database

Research Area

DATA IMAGES
GTX15669_285_ICC-IF_19011519_761.jpg

GTX15669 ICC/IF Image

ICC/IF analysis of Raji cells using GTX15669 CD58 antibody [TS2/9]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:20 overnight at 4°C

GTX15669_103_FACS_19011519_105.jpg

GTX15669 FACS Image

FACS analysis of PBMC cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue).
Dilution : 0.5 ug/test incubated for 30 min at 4°C

GTX15669_101_FACS_19011519_126.jpg

GTX15669 FACS Image

FACS analysis of BAF-3 cells using GTX15669 CD58 antibody [TS2/9] compared to an isotype control (blue).
Dilution : 0.25 ug/test for 60 min at room temperature
Fixation : 2% paraformaldehyde

GTX15669_1514_WB_19011519_925.jpg

GTX15669 WB Image

WB analysis of 25 ug of Raji (Lane 1), Jurkat (Lane 2), and BAF-3 cell lysates (Lane 3) using GTX15669 CD58 antibody [TS2/9].
Dilution : 1:100

SDS
PBS.pdf
Package List Price ($)
$ 319