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CD59 antibody [8D2B8]

Anti-CD59 antibody [8D2B8] used in Western Blot (WB). GTX60658
Anti-CD59 antibody [8D2B8] used in IHC (Paraffin sections) (IHC-P). GTX60658
Anti-CD59 antibody [8D2B8] used in Flow cytometry (FACS). GTX60658
Anti-CD59 antibody [8D2B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60658
Anti-CD59 antibody [8D2B8] used in ELISA (ELISA). GTX60658
Anti-CD59 antibody [8D2B8] used in IHC (Paraffin sections) (IHC-P). GTX60658
Anti-CD59 antibody [8D2B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60658
Anti-CD59 antibody [8D2B8] used in Flow cytometry (FACS). GTX60658

Cat. No. GTX60658

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8D2B8

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all CD59 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/50 -1/200
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human CD59 (AA: 31-111) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2887994

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD59 molecule (CD59 blood group) , 16.3A5 , 1F5 , EJ16 , EJ30 , EL32 , G344 , HRF-20 , HRF20 , MAC-IP , MACIF , MEM43 , MIC11 , MIN1 , MIN2 , MIN3 , MIRL , MSK21 , p18-20

Cellular Localization

Secreted

Background

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD59 antibody [8D2B8] used in Western Blot (WB). GTX60658

GTX60658 WB Image

WB analysis of HEK293 (1) and CD59 (AA: 31-111)-hIgGFc transfected HEK293 (2) cell lysate using GTX60658 CD59 antibody [8D2B8].

Anti-CD59 antibody [8D2B8] used in IHC (Paraffin sections) (IHC-P). GTX60658

GTX60658 IHC-P Image

IHC-P analysis of bladder cancer tissue using GTX60658 CD59 antibody [8D2B8].

Anti-CD59 antibody [8D2B8] used in Flow cytometry (FACS). GTX60658

GTX60658 FACS Image

FACS analysis of HeLa cells using GTX60658 CD59 antibody [8D2B8].
Green : CD59
Red : negative control

Anti-CD59 antibody [8D2B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60658

GTX60658 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60658 CD59 antibody [8D2B8].
Green : CD59
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-CD59 antibody [8D2B8] used in ELISA (ELISA). GTX60658

GTX60658 ELISA Image

ELISA analysis of antigen using GTX60658 CD59 antibody [8D2B8].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-CD59 antibody [8D2B8] used in IHC (Paraffin sections) (IHC-P). GTX60658

GTX60658 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60658 CD59 antibody [8D2B8].

Anti-CD59 antibody [8D2B8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60658

GTX60658 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX60658 CD59 antibody [8D2B8].
Green : CD59
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-CD59 antibody [8D2B8] used in Flow cytometry (FACS). GTX60658

GTX60658 FACS Image

The data was published in the journal PLoS One in 2017.PMID: 29121670

REFERENCE

Application Reference

Viswanathan K et al. PLoS One 2017; 12 (11) : e0187899 Quantitative membrane proteomics reveals a role for tetraspanin enriched microdomains during entry of human cytomegalovirus.
Application : FACS
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409