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United States (US)

CD59 antibody [MACIF/629]

GTX34532_20170712_IHC-P_18121410_177.jpg
GTX34532_20170712_IHC-P_1_18121410_279.jpg

Cat No. GTX34532

Host Mouse
Clonality Monoclonal
Clone Name MACIF/629
Isotype IgG1
Application IHC-P
Reactivity Human
APPLICATION

Application Note

IHC-P:1-2ug/ml for 30 minutes at RT.

Calculated MW

14 kDa. ( Note )

Specificity/Sensitivity

Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human CD59 protein

Purification

Ab purified from Bioreactor Concentrate

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd59 Molecule (Cd59 Blood Group),16.3A5,1F5,Ej16,Ej30,El32,G344,Hrf-20,Hrf20,Mac-Ip,Macif,Mem43,Mic11,Min1,Min2,Min3,Mirl,Msk21,P18-20,Cd59

Background

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX34532_20170712_IHC-P_18121410_177.jpg

GTX34532 IHC-P Image

Formalin-fixed, paraffin-embedded human Tongue stained with CD59 Monoclonal Antibody (MACIF/629)

GTX34532_20170712_IHC-P_1_18121410_279.jpg

GTX34532 IHC-P Image

Formalin-fixed, paraffin-embedded human Tonsil stained with CD59 Monoclonal Antibody (MACIF/629)

Package List Price ($)
$ 329