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CD59 antibody [MEM-43] (Azide free)

Anti-CD59 antibody [MEM-43] (Azide free) used in Flow cytometry (FACS). GTX74620

Cat. No. GTX74620

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-43

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human
Package
500 μg ($659)
See all CD59 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 1/5-1/10
IP Assay dependent
ELISA Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Thymocytes and T lymphocytes.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_376378

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD59 molecule (CD59 blood group) , 16.3A5 , 1F5 , EJ16 , EJ30 , EL32 , G344 , HRF-20 , HRF20 , MAC-IP , MACIF , MEM43 , MIC11 , MIN1 , MIN2 , MIN3 , MIRL , MSK21 , p18-20

Cellular Localization

Secreted

Background

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD59 antibody [MEM-43] (Azide free) used in Flow cytometry (FACS). GTX74620

GTX74620 FACS Image

FACS analysis of human peripheral blood lymphocytes using GTX74620 CD59 antibody [MEM-43] (Azide free).

REFERENCE

Clone Reference

Lee TC et al. PLoS One 2014; 9 (11):e111390 Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells.
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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 659
Custom Conjugations