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CD59 antibody [YTH53.1]

Anti-CD59 antibody [YTH53.1] used in IHC (Paraffin sections) (IHC-P). GTX76425
Anti-CD59 antibody [YTH53.1] used in Flow cytometry (FACS). GTX76425

Cat. No. GTX76425

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 YTH53.1

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, Blocking, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all CD59 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1/5-1/10
IHC-Fr Assay dependent
FACS 1/50-1/100
Blocking Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

IHC-P
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Rat anti Human CD59 antibody, clone YTH53.1 blocks the normal function of CD59 (Walsh et al. 1991).
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human Peripheral Blood T cells.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_377340

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD59 molecule (CD59 blood group) , 16.3A5 , 1F5 , EJ16 , EJ30 , EL32 , G344 , HRF-20 , HRF20 , MAC-IP , MACIF , MEM43 , MIC11 , MIN1 , MIN2 , MIN3 , MIRL , MSK21 , p18-20

Cellular Localization

Secreted

Background

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD59 antibody [YTH53.1] used in IHC (Paraffin sections) (IHC-P). GTX76425

GTX76425 IHC-P Image

IHC-P analysis of human placenta tissue using GTX76425 CD59 antibody [YTH53.1].

Anti-CD59 antibody [YTH53.1] used in Flow cytometry (FACS). GTX76425

GTX76425 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX76425 CD59 antibody [YTH53.1].

REFERENCE

Application Reference

Gelber SE et al. J Bacteriol 2008; 190 (11) : 3896-903 Functional and phylogenetic characterization of Vaginolysin, the human-specific cytolysin from Gardnerella vaginalis.
Application : Blocking
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399