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CD59 antibody

Anti-CD59 antibody used in IHC (Paraffin sections) (IHC-P). GTX130096
Anti-CD59 antibody used in Western Blot (WB). GTX130096

Cat No. GTX130096

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

14 kDa. ( Note )

Positive Control

K562 , HL-60

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1975 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human CD59. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD59 molecule (CD59 blood group) , 16.3A5 , 1F5 , EJ16 , EJ30 , EL32 , G344 , HRF-20 , HRF20 , MAC-IP , MACIF , MEM43 , MIC11 , MIN1 , MIN2 , MIN3 , MIRL , MSK21 , p18-20

Cellular Localization

Cell membrane , GPI-anchor , Secreted

Background

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-CD59 antibody used in IHC (Paraffin sections) (IHC-P). GTX130096

GTX130096 IHC-P Image

CD59 antibody detects CD59 protein at cell membrane in human cervical carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
CD59 antibody (GTX130096) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD59 antibody used in Western Blot (WB). GTX130096

GTX130096 WB Image

CD59 antibody detects CD59 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD59 antibody (GTX130096) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169