APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.95 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CD59. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886763
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD59 molecule (CD59 blood group) , 16.3A5 , 1F5 , EJ16 , EJ30 , EL32 , G344 , HRF-20 , HRF20 , MAC-IP , MACIF , MEM43 , MIC11 , MIN1 , MIN2 , MIN3 , MIRL , MSK21 , p18-20
Cellular Localization
Secreted
Background
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX132874 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with CD59 (GTX132874) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
|
|
|
GTX132874 ICC/IF Image
CD59 antibody detects CD59 protein at cell membrane and secreted by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD59 stained by CD59 antibody (GTX132874) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
|
REFERENCE
There are currently no references for CD59 antibody (GTX132874). Be the first to share your publications with this product.
REVIEW
CD59 antibody
Cat. No. GTX132874
|
Rating
|
|
( Average 4 based on 1 users reviews)
|
Application
|
Western Blot(WB)
|
|
( Average 4 based on 1 users reviews)
|
Date :
Anonymous submitted on 04-Jul-2023
Application Tested :
WB
Sample Species :
Hu
Sample :
RPE1 cells
Amount used :
10μg
Blocking :
5% non-fat milk in PBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, >12Hr
