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CD6 antibody [SPM547]

Anti-CD6 antibody [SPM547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34537
Anti-CD6 antibody [SPM547] used in IHC (Paraffin sections) (IHC-P). GTX34537

Cat. No. GTX34537

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM547

Isotype

 IgG1

Application

 ICC/IF, IHC-P

Reactivity

 Human
Package
100 μg ($529)
See all CD6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

72 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human recombinant CD6 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD6 , CD6 molecule , TP120

Cellular Localization

Cell membrane

Background

This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-CD6 antibody [SPM547] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34537

GTX34537 ICC/IF Image

ICC/IF analysis of MOLT-4 cells using GTX34537 CD6 antibody [SPM547].
Green : Primary antibody
Red : nucleus

Anti-CD6 antibody [SPM547] used in IHC (Paraffin sections) (IHC-P). GTX34537

GTX34537 IHC-P Image

IHC-P analysis of tonsil tissue using GTX34537 CD6 antibody [SPM547].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529