CD63 antibody [GT368]

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with CD63 antibody [GT368] (GTX646552) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Sandwich ELISA detection of recombinant HEK293 expressed Human CD63 (ECD) protein, human Fc tag (GTX144107-pro) using antibodies as below.

Capture: CD63 antibody [GT368] (GTX646552) (5 μg/mL)
Detection: CD63 antibody [HL5890] (GTX647214) (1 μg/mL)

Sandwich ELISA detection of recombinant HEK293 expressed Human CD63 (ECD) protein, human Fc tag (GTX144107-pro) using antibodies as below.

Capture: CD63 antibody [GT368] (GTX646552) (5 μg/mL)
Detection: CD63 antibody [HL5889] (GTX647213) (1 μg/mL)

Indirect ELISA analysis was performed by coating the plate with recombinant HEK293 expressed Human CD63 (ECD) protein, human Fc tag (GTX144107-pro) (0.82-52.63 nM). Coated protein was probed with CD63 antibody [GT368] (GTX646552) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Boiled and unboiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD63 antibody [GT368] (GTX646552) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data are based on Human Protein Atlas program.