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United States (US)

CD63 antibody [MEM-259]

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Cat No. GTX28219

Host Mouse
Clonality Monoclonal
Clone Name MEM-259
Isotype IgG1
Application WB, ICC/IF, IHC-P, FACS, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 10 μg/ml
FACS 2 μg/ml
IP Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

The antibody MEM-259 reacts with CD63 (Melanoma 1 antigen), a 40-60 kDa glycoprotein (tetraspans family, TM4SF). CD63 is translocated to the cell surface upon activation of platelets, endothelial cells, and granulocytes (activation marker); it is also expressed on monocytes/macrophages and endothelium.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Tissue / cell preparation (Human). (T cell line HPB-ALL)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LAMP-3, ME491, MLA1, OMA81H, TSPAN30

Background

CD63 is intracellular lysosomal/endosomal/granule protein that is translocated to cell surface upon activation of platelets, endothelial cells, and granulocytes (activation marker). CD63 is also expressed on monocytes/macrophages and endothelium. Its cellular function has not been exactly elucidated. CD63 is thought to be associated with the early stages of melanoma tumor progression (in regulation of motility and adhesion of melanoma cells).

Database

Research Area

DATA IMAGES
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GTX28219 FACS Image

Flow cytometry analysis of peripheral blood lymphocytes from a patient with allergy to bee venom after stimulation with bee venom, stained with anti-human CD63 (MEM-259) FITC (GTX18235).

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GTX28219 ICC/IF Image

Immunofluorescence staining of CD63 in human primary fibroblasts using anti-CD63 (MEM-259)(GTX28219)(green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

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GTX28219 ICC/IF Image

Immunofluorescence staining of human skin fibroblasts with anti-CD63 (MEM-259 (GTX28219); green) after co-incubation of living cells with human Transferrin - Dyomics 547 (red); cell nuclei stained with DAPI (blue).

CD63-antibody-MEM-259__GTX28219-2_18121409_591.gif

GTX28219 ICC/IF Image

Immunofluorescence staining of CD63 in human HeLa cell line using anti-CD63 (MEM-259 (GTX28219); green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

REFERENCE
Package List Price ($)
$ 319