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CD63 antibody [MEM-259]

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219
Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219
Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219
Anti-CD63 antibody [MEM-259] used in Flow cytometry (FACS). GTX28219

Cat. No. GTX28219

Reference 12

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 MEM-259

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, IP, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all CD63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 10 μg/ml
IHC-P 10 μg/ml
FACS 2 μg/ml
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

HPB-ALL T cell line

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_374496

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD63 , CD63 molecule , LAMP3 , ME491 , MLA1 , OMA81H , TSPAN30

Cellular Localization

Cell membrane,Lysosome membrane,Late endosome membrane,Endosome,Multivesicular body,Melanosome

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219

GTX28219 ICC/IF Image

ICC/IF analysis of human primary fibroblasts using GTX28219 CD63 antibody [MEM-259].
Green : Primary antibody
Red : Actin
Blue : DAPI

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219

GTX28219 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX28219 CD63 antibody [MEM-259].
Green : Primary antibody
Red : Actin
Blue : DAPI

Anti-CD63 antibody [MEM-259] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28219

GTX28219 ICC/IF Image

ICC/IF analysis of human skin fibroblasts using GTX28219 CD63 antibody [MEM-259].
Green : Primary antibody
Red : Transferrin
Blue : DAPI

Anti-CD63 antibody [MEM-259] used in Flow cytometry (FACS). GTX28219

GTX28219 FACS Image

FACS analysis of IgE-activated peripheral blood using GTX28219 CD63 antibody [MEM-259].

REFERENCE

Application Reference

Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells
Application : ICC/IF
Lin SJ et al. Nat Commun 2023; 14 (1) : 7859 Endosomal Arl4A attenuates EGFR degradation by binding to the ESCRT-II component VPS36.
Lanna A et al. Nat Cell Biol 2022; 24 (10) : 1461-1474 An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
Application : ELISA
Reactivity : Human
Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4) : e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.

Clone Reference

Huang C.C. et al. Microfluidics and Nanofluidics 2021; 25 A miniaturized, DNA-FET biosensor-based microfluidic system for quantification of two breast cancer biomarkers.
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REVIEW

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Application Reference
Afrodit? N?meth et al. Research Square 2023; Extracellular vesicles promote migration despite vemurafenib treatment in malignant melanoma cells
Application : ICC/IF
Lin SJ et al. Nat Commun 2023; 14 (1):7859 Endosomal Arl4A attenuates EGFR degradation by binding to the ESCRT-II component VPS36.
Lanna A et al. Nat Cell Biol 2022; 24 (10):1461-1474 An intercellular transfer of telomeres rescues T cells from senescence and promotes long-term immunological memory.
Application : ELISA
Reactivity : Human
Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4):e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.
Peng X et al. J Exp Clin Cancer Res 2021; 40 (1):183 LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation.
Application : ICC/IF
Reactivity : Human
Peng X et al. Biochim Biophys Acta Mol Cell Res 2021; 1868 (1):118857 IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.
Application : WB, ICC/IF
Reactivity : Human
Yang L et al. Mol Cancer 2019; 18 (1):78 Long non-coding RNA HOTAIR promotes exosome secretion by regulating RAB35 and SNAP23 in hepatocellular carcinoma.
Raulf N et al. Eur J Cancer 2018; 102:52-68 Annexin A1 regulates EGFR activity and alters EGFR-containing tumour-derived exosomes in head and neck cancers.
Application : WB
Reactivity : Human
Clone Reference
Lenzini S et al. ACS Nano 2021; 15 (11):17439-17452 Cell-Matrix Interactions Regulate Functional Extracellular Vesicle Secretion from Mesenchymal Stromal Cells.
Huang C.C. et al. Microfluidics and Nanofluidics 2021; 25 A miniaturized, DNA-FET biosensor-based microfluidic system for quantification of two breast cancer biomarkers.
Jakhria T et al. J Biol Chem 2014; β2-microglobulin Amyloid Fibrils are Nanoparticles that Disrupt Lysosomal Membrane Protein Trafficking and Inhibit Protein Degradation by Lysosomes.
Schmidt H et al. BMC Immunol 2009; 10:41 Enrichment and analysis of secretory lysosomes from lymphocyte populations.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399