WB:0.5-1ug/ml, IHC-P:0.5-1ug/ml for 30 minutes at RT, FACS:0.5-1ug/million cells in 0.1ml.
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Smooth plasma membrane fraction of MeWo cells
Ab purified from Bioreactor Concentrate by Protein A/G
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
GTX34538 IHC-P Image
Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Monoclonal Antibody (NKI/C3) (AEC Chromogen)
GTX34538 FACS Image
Flow Cytometry of human CD63 on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (NKI/C3).
GTX34538 IHC-P Image
Formalin-fixed, paraffin-embedded Mouse Spleen stained with CD63 Monoclonal Antibody (NKI/C3) (DAB Chromogen)
GTX34538 WB Image
Western Blot of human spleen Lysate with CD63 Monoclonal Antibody (NKI/C3)