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United States (US)

CD63 antibody [NKI/C3]

GTX34538_20170712_IHC-P_18121410_569.jpg
GTX34538_20170712_FACS_18121410_528.jpg
GTX34538_20170712_IHC-P_1_18121410_306.jpg
GTX34538_20170712_WB_18121410_268.jpg

Cat No. GTX34538

Host Mouse
Clonality Monoclonal
Clone Name NKI/C3
Isotype IgG1
Application WB, IHC-P, FACS
Reactivity Human, Mouse
APPLICATION

Application Note

WB:0.5-1ug/ml, IHC-P:0.5-1ug/ml for 30 minutes at RT, FACS:0.5-1ug/million cells in 0.1ml.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Smooth plasma membrane fraction of MeWo cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Cd63 Molecule,Lamp-3,Me491,Mla1,Oma81H,Tspan30,Cd63

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES
GTX34538_20170712_IHC-P_18121410_569.jpg

GTX34538 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Monoclonal Antibody (NKI/C3) (AEC Chromogen)

GTX34538_20170712_FACS_18121410_528.jpg

GTX34538 FACS Image

Flow Cytometry of human CD63 on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (NKI/C3).

GTX34538_20170712_IHC-P_1_18121410_306.jpg

GTX34538 IHC-P Image

Formalin-fixed, paraffin-embedded Mouse Spleen stained with CD63 Monoclonal Antibody (NKI/C3) (DAB Chromogen)

GTX34538_20170712_WB_18121410_268.jpg

GTX34538 WB Image

Western Blot of human spleen Lysate with CD63 Monoclonal Antibody (NKI/C3)

Package List Price ($)
$ 329