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CD63 antibody [NKI/C3]

Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538
Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538
Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538
Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538

Cat No. GTX34538

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NKI/C3

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($339)
See all CD63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Smooth plasma membrane fraction of MeWo cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD63 , CD63 molecule , LAMP3 , ME491 , MLA1 , OMA81H , TSPAN30

Cellular Localization

Cell membrane,Lysosome membrane,Late endosome membrane,Endosome,Multivesicular body,Melanosome

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538

GTX34538 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34538 CD63 antibody [NKI/C3].

Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538

GTX34538 FACS Image

FACS analysis of MCF-7 cells using GTX34538 CD63 antibody [NKI/C3].
Black : Blank
Green : Isotype Control
Red : Primary antibody

Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538

GTX34538 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX34538 CD63 antibody [NKI/C3].

Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538

GTX34538 FACS Image

FACS analysis of PFA-fixed U87MG cells using GTX34538 CD63 antibody [NKI/C3].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339