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CD63 antibody [NKI/C3]

Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538
Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538
Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538
Anti-CD63 antibody [NKI/C3] used in Western Blot (WB). GTX34538

Cat No. GTX34538

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 NKI/C3

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($429)
See all CD63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT
FACS Assay dependent

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

Specificity/Sensitivity

This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

Smooth plasma membrane fraction of MeWo cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Cd63 Molecule , Lamp-3 , Me491 , Mla1 , Oma81H , Tspan30 , Cd63

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538

GTX34538 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Monoclonal Antibody (NKI/C3) (AEC Chromogen)

Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538

GTX34538 FACS Image

Flow Cytometry of human CD63 on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (NKI/C3).

Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538

GTX34538 IHC-P Image

Formalin-fixed, paraffin-embedded Mouse Spleen stained with CD63 Monoclonal Antibody (NKI/C3) (DAB Chromogen)

Anti-CD63 antibody [NKI/C3] used in Western Blot (WB). GTX34538

GTX34538 WB Image

Western Blot of human spleen Lysate with CD63 Monoclonal Antibody (NKI/C3)

REFERENCE

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Package List Price ($)
$ 429