APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
Product Note
This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Smooth plasma membrane fraction of MeWo cells
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
CD63 , CD63 molecule , LAMP3 , ME491 , MLA1 , OMA81H , TSPAN30
Cellular Localization
Cell membrane,Lysosome membrane,Late endosome membrane,Endosome,Multivesicular body,Melanosome
Background
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]
Database
Research Area
DATA IMAGES
![Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538](/templates/default/js/lightbox/images/loading.gif "Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538")
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GTX34538 FACS Image
FACS analysis of MCF-7 cells using GTX34538 CD63 antibody [NKI/C3].
Black : Blank
Green : Isotype Control
Red : Primary antibody
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GTX34538 FACS Image
FACS analysis of PFA-fixed U87MG cells using GTX34538 CD63 antibody [NKI/C3].
Blue : Primary antibody
Red : Isotype control
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GTX34538 IHC-P Image
IHC-P analysis of mouse spleen tissue using GTX34538 CD63 antibody [NKI/C3].
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GTX34538 IHC-P Image
IHC-P analysis of human melanoma tissue using GTX34538 CD63 antibody [NKI/C3].
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REFERENCE
There are currently no references for CD63 antibody [NKI/C3] (GTX34538). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for CD63 antibody [NKI/C3] (GTX34538). Be the first to share your experience with this product.
![Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538](/upload/website/prouct_img/small/GTX34538/GTX34538_20200115_FACS_1548_w_23060801_619.webp "Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538](/upload/website/prouct_img/small/GTX34538/GTX34538_20200115_FACS_1760_w_23060801_217.webp "Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538](/upload/website/prouct_img/small/GTX34538/GTX34538_20200115_IHC-P_1635_w_23060801_124.webp "Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538](/upload/website/prouct_img/small/GTX34538/GTX34538_20200115_IHC-P_733_w_23060801_146.webp "Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538](/upload/website/prouct_img/normal/GTX34538/GTX34538_20200115_FACS_1548_w_23060801_619.webp "Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538](/upload/website/prouct_img/normal/GTX34538/GTX34538_20200115_FACS_1760_w_23060801_217.webp "Anti-CD63 antibody [NKI/C3] used in Flow cytometry (FACS). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538](/upload/website/prouct_img/normal/GTX34538/GTX34538_20200115_IHC-P_1635_w_23060801_124.webp "Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538")
![Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538](/upload/website/prouct_img/normal/GTX34538/GTX34538_20200115_IHC-P_733_w_23060801_146.webp "Anti-CD63 antibody [NKI/C3] used in IHC (Paraffin sections) (IHC-P). GTX34538")