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CD63 antibody [SPM524]

Anti-CD63 antibody [SPM524] used in IHC (Paraffin sections) (IHC-P). GTX34539
Anti-CD63 antibody [SPM524] used in IHC (Paraffin sections) (IHC-P). GTX34539

Cat. No. GTX34539

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM524

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($529)
See all CD63 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

26 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Smooth plasma membrane fraction of MeWo cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD63 , CD63 molecule , LAMP3 , ME491 , MLA1 , OMA81H , TSPAN30

Cellular Localization

Cell membrane,Lysosome membrane,Late endosome membrane,Endosome,Multivesicular body,Melanosome

Background

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Database

Research Area

DATA IMAGES

Anti-CD63 antibody [SPM524] used in IHC (Paraffin sections) (IHC-P). GTX34539

GTX34539 IHC-P Image

IHC-P analysis of human prostate tissue using GTX34539 CD63 antibody [SPM524].

Anti-CD63 antibody [SPM524] used in IHC (Paraffin sections) (IHC-P). GTX34539

GTX34539 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34539 CD63 antibody [SPM524].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529