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CD64 antibody [10.1] (Low endotoxin, azide free)

Anti-CD64 antibody [10.1] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX76486
Anti-CD64 antibody [10.1] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX76486

Cat. No. GTX76486

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10.1

Isotype

 IgG1

Application

 ICC/IF, FACS, Blocking, Neutralizing/Inhibition

Reactivity

 Human, Baboon, Cynomolgus monkey, Rhesus Monkey
Package
100 μg ($399)
See all CD64 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF Assay dependent
FACS 1/50-1/200
Blocking Assay dependent
Neutralizing/Inhibition Assay dependent

Note :

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human monocytes.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

RRID

AB_377385

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Fc fragment of IgG receptor Ia , CD64 , CD64A , FCRI , IGFR1

Cellular Localization

Cell membrane

Background

This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-CD64 antibody [10.1] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX76486

GTX76486 FACS Image

FACS analysis of peripheral blood monocytes using GTX76486 CD64 antibody [10.1] (Low endotoxin, azide free).

Anti-CD64 antibody [10.1] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX76486

GTX76486 FACS Image

FACS analysis of human peripheral blood monocytes using GTX76486 CD64 antibody [10.1] (Low endotoxin, azide free).

REFERENCE

Application Reference

Li M et al. PLoS One 2018; 13 (7) : e0200478 Dengue immune sera enhance Zika virus infection in human peripheral blood monocytes through Fc gamma receptors.
Richards JO et al. Mol Cancer Ther 2008; 7 (8) : 2517-27 Optimization of antibody binding to FcgammaRIIa enhances macrophage phagocytosis of tumor cells.
Application : Blocking
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations