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CD66b antibody [80H3]

Cat. No. GTX75908

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 80H3

Isotype

 IgG1

Application

 WB, IHC-Fr, FACS, Functional Assay

Reactivity

 Human
Package
40 μg ($169)
See all CD66b products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-Fr 1/25-1/100
FACS 1/50-1/100
Functional Assay Assay dependent

Note :

IHC-Fr
The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections.

FACS
Use 10μl of the suggested working dilution to label 5 x 105 cells or 100μl whole blood.
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Bone marrow

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Human leucocytes from individuals with CML.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_377113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carcinoembryonic antigen related cell adhesion molecule 8 , CD66b , CD67 , CGM6 , NCA-95

Cellular Localization

Cell membrane

Database

Research Area

REFERENCE

Clone Reference

Yu Y et al. J Thromb Haemost 2018; (Epub) Extracellular vesicles from human saliva promote haemostasis by delivering coagulant tissue factor to activated platelets.
Eken C et al. Immunobiology 2013; 218 (3):382-92 Ectosomes of polymorphonuclear neutrophils activate multiple signaling pathways in macrophages.
Eken C et al. J Biol Chem 2010; 285 (51):39914-21 Ectosomes released by polymorphonuclear neutrophils induce a MerTK-dependent anti-inflammatory pathway in macrophages.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 169