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CD66e antibody [HL2467]

Recombinant antibody Orthogonal
Anti-CD66e antibody [HL2467] used in IHC (Paraffin sections) (IHC-P). GTX638816
Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816
Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816

Cat. No. GTX638816

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2467

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all CD66e products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human CD66e.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carcinoembryonic antigen related cell adhesion molecule 5 , CD66e , CEA

Cellular Localization

Cell membrane,Apical cell membrane,Cell surface

Background

This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Database

Research Area

DATA IMAGES

Anti-CD66e antibody [HL2467] used in IHC (Paraffin sections) (IHC-P). GTX638816

GTX638816 IHC-P Image

CD66e antibody [HL2467] detects CD66e protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CD66e stained by CD66e antibody [HL2467] (GTX638816) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816

GTX638816 WB Image

Orthogonal

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD66e antibody [HL2467] (GTX638816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-CD66e antibody [HL2467] used in Western Blot (WB). GTX638816

GTX638816 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD66e antibody [HL2467] (GTX638816) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations